View at Google Scholar A. There are few reports, which utilized the vast potential of these frying oils for biosurfactant production. Characterization of the biosurfactant revealed that, it is a lipopeptide with chemical composition of protein There has been postulated that sequences conserved in some promoters regions of RhlR and LasR-regulated genes are responsible by these regulation [ 75 ]. Surface tension of supernatants obtained with sugar cane molasses or glycerol as substrates. This could be due to the wide variability of biosurfactants metabolism with strain and carbon source used [ 68 ]. Characterization of Biosurfactant Biochemical Composition of Biosurfactant Carbohydrate content of the biosurfactant was determined by the phenol—sulfuric acid method [ 14 ] using d -glucose as a standard.

Lipopeptides, such as surfactins, are particularly interesting because of their high surface activities and antibiotic potential. There are two known conventional QS systems in P. It was shown that this strain is capable of utilizing up to four-ring PAHs but not hexadecane and octadecane as a sole carbon and energy source, and the authors identified the presence of both tension-active and emulsifying activities, suggesting that IR1 produces biosurfactants on both water miscible and immiscible substrates. Major types of biosurfactants. June 24th Reviewed:

The bacterium identification was conducted by the molecular method depending on gene phylogenetic approximation. Marty P, Martin Y.

biosurfactant production thesis

In biosurfsctant to complete the nature of biosurfactants produced by the three lactobacilli strains, the presence of phosphates in their composition was sought. Oilfield emulsions are formed at various stages of petroleum exploration, production and oil recovery and processing, and represent one of the major problems for the petroleum industry [ 39 ], which requires a de-emulsification process in order to recover oil from theses emulsions.

Oil mobilization was the main cause for its removal, which was triggered by the reduction of surface and interfacial tensions, rather than oil solubilization or emulsification. More recently, other methods for biosurfactants recovery have been reported, including foam fractionation, ultrafiltration and adsorption-desorption on polystyrene resins and ion exchange chromatography.


Biosurfactants: Production and Applications

Several studies have aimed to optimize the biosurfactant production process by changing the variables that influence the type and amount of biosurfactant produced by a microorganism. These findings clearly indicate the other factors are involved in the expression of QS-regulated genes.

High hydrophobicity and solid-water distribution ratios of some pollutants result in their interaction with non-aqueous phases and soil organic matter. A significant reduction in the surface tension of V. Surfactants have potential application for oil recovery from petroleum tank bottom sludges and facilitating heavy crude transport through pipelines.

View at Google Scholar G. Metabolism and gene regulation of rhamnolipid production Biosurfactants are considered ideal for environmental application, due to their numerous advantages over their chemical counterparts, such as biodegradability and less toxicity [ 60 ].

biosurfactant production thesis

In addition, the results in Table 2 show the strain V. Please review our privacy policy. The presence of biosurfactants in the solution was confirmed based on a decrease in the value of surface tension of the supernatants against the control distilled water.

Protein content was determined by the method of Lowry et al. Login to your personal dashboard for more detailed statistics on your publications. The increase in extra-cellular biosurfactant concentration might be the result of the cell-bound biosurfactant molecules released into the broth medium Deziel et al.

Thavasi1 V.

Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources

A unique biodegradation pattern of the oil spilled during the Introduction The surface-active compounds commonly used in many industries are chemically synthesized; they are widely used in almost every sector of recent industry Samadi et al. Over 21, IntechOpen readers like this topic Help us write another book on this subject and reach those readers Suggest a book topic Books open for submissions. The expansion in environmental carefulness has led to serious consideration of biological surfactants as the most promising alternative to existing product Henkel et al.


In this light, several studies have been carried out using sugar cane molasses as substrate to increase the production yields of biosurfactants [ 3233 ].

When the substrates are considered, sugar cane molasses seems to be the best among the studied substrates. Emulsification Activity E24 The emulsifying capacity was evaluated by an emulsification index E Support Center Support Center.

Time course of biosurfactant production kinetics in optimal medium The kinetics of biosurfactant was followed in a modified MS medium at optimum conditions. Emulsification activity found with the biosurfactant against different hydrocarbons showed its possible application in bioremediation of environments polluted with various hydrocarbons. Potential commercial applications of microbial surfactants. In gidA mutants, the levels of rhlR mRNA are similar to the levels in wild-type, whereas rhlA mRNA levels biosurfactany significantly decreased, suggesting that the rhamnolipid production is controlled via posttranscriptional modulation of RhlR levels by GidA [ 82 ].

The possibility of economical biosurfactant production to reduce pollution was caused by wastes. Characterization of Biosurfactant Biochemical Composition of Biosurfactant Carbohydrate content of the biosurfactant was determined by the phenol—sulfuric acid method [ 14 ] using d -glucose as a standard.

Biosurfactant concentration in the culture broth was estimated according to the procedure described by Li et al. Subramanyam Nambaru2 S.

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