THESIS ON SCRUB TYPHUS

Abstract Introduction Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections. In this study an attempt has been made to know the seropositivity in clinically suspected scrub typhus patients. The IFA slide presents antigens from only 3 serotypes namely Karp, Kato and Gilliam, thus unable to detect other antigenically variable strains of Orientia tsutsugamushi affecting the sensitivity of the test [ 14 ]. Expert Rev Anti Infect Ther. The test is widely in use as it is cheap, easily available and does not require technical expertise.

The current gold standard for serology is Indirect Immunofluorescence Antibody IFA test which uses a fluorescence labelled anti-human immunoglobulin to detect antibodies in patients serum that have bound to immobilised bacterial antigen that is coated on the IFA slide. High-level expression of a kilodalton protein gene bor56 of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. The diagnosis of scrub typhus is generally made by the history and clinical presentation. The current gold standard is imperfect as it is expensive requiring a fluorescence microscope. An emerging infectious threat. J Assoc Physicians India. The IFA slide presents antigens from only 3 serotypes namely Karp, Kato and Gilliam, thus unable to detect other antigenically variable strains of Orientia tsutsugamushi affecting the sensitivity of the test [ 14 ].

Acknowledgement for financial support and specific contribution only: The test was standardised with serum samples from healthy blood donors. The Immunofluorescence antibody IFA test has its limitations in terms of cost and expertise required. However, it lacks specificity and sensitivity [ 15 ].

thesis on scrub typhus

Investigation of an outbreak of scrub typhus in the himalayan region of India. Nested PCR for detection of 56 kDa gene specific for Orientia tsutsugamushi with good sensitivity of and specificity may be recommended in laboratories with molecular lab facility [ 14 ]. The mite acts as both vector and reservoir of the bacteria and efficiently transmits to its off springs through transovarian transmission.

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The IFA slide presents antigens from only 3 serotypes namely Karp, Kato and Gilliam, thus unable to detect other antigenically variable strains of Orientia tsutsugamushi affecting the sensitivity of the test [ 14 ]. The organism is also extremely dangerous requiring BSL—III facility for its culture which is impractical in most laboratories in India [ 15 ]. Results After screening patients with acute febrile illness with a battery of diagnostic tests such as Para F for malaria, Widal tests, ICT for dengue and Ig M ELISA for leptospirosis, 63 samples reacted positively for above mentioned tests and were excluded.

The medical fraternity needs to understand the pitfalls of currently available Weil-Felix test which is still common in India and must include tests with better performance like IgM ELISA and nested PCR in the diagnostic algorithm of scrub typhus to reduce the burden of the disease.

Abstract Introduction Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections.

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

Indian J Med Microbiol. Asian J Pharm Clin Res. The patients age varied from 10 months to 80 years were taken for the study. With either delay in diagnosis or administration of inappropriate antimicrobial therapy can lead to severe complications such as Acute Respiratory Distress Syndrome ARDSseptic shock and multisystem organ failure often causing death in om.

Ann Indian Acad Neurol. Find articles by Madhan Sugumar.

Introduction Scrub typhus has been one of the most covert emerging and re-emerging Rickettsial yhesis with increasing trend in incidences of the disease worldwide including India.

Indian J Med Res. Though it is rationally considered as disease found in rural areas, it is also well described from urban areas like Delhi and Chennai infecting people, as the tiny mite islands are seen in the vegetation around the dwellings. The vast variability and common clinical manifestations of the disease which is similar to other febrile illnesses makes the clinical diagnosis challenging.

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It is a prospective study which was carried out between January to June 3 years 6 monthsin a typhuw care hospital in Puducherry, South India. Typhus fever in Pondicherry. Outbreak of scrub typhus in the North East Himalayan region-Sikkim: The most common diagnostic test in India is the Weil-Felix test.

thesis on scrub typhus

In this study an attempt has been made to know the seropositivity in clinically suspected scrub typhus patients. An emerging infectious threat. This again emphasises the fact that Weil-Felix test is not a sensitive test for diagnosis of scrub typhus.

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

Published online Nov 1. Scrub typhus has been one of the most covert emerging and re-emerging Rickettsial infections with increasing trend in incidences of the disease worldwide including India. J Assoc Physicians India. The commercially available IFA kits cannot be afforded in resource poor settings. Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order.

Materials and Methods It is a prospective study which was carried out between January to June 3 years 6 monthsin a tertiary care hospital in Puducherry, South India. After screening patients with acute febrile illness with a battery of diagnostic tests such as Para F for malaria, Widal tests, ICT for dengue and Ig M ELISA for leptospirosis, 63 samples reacted positively for above mentioned tests and were excluded.

thesis on scrub typhus

A study was conducted in Delhi among the febrile paediatric patients which did not have definitive diagnosis.

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